Extracting Dna From Onions

Extracting DNA from Onions:

By Arun R Shrivats

Observations:

Qualitative Observations:

Qualitative Obervations of DNA Extraction Process in Onions

Stage in DNA Extraction Process Qualitative Observations

1. After addition of Dish Soap * Foam formed at the top

* Greenish yellow

* Fluidy

* Opaque

* Odour: Mixture of onion and soap

2. After 5 minutes * Foam formed t the top

* Lime colour

* Fluidy

* Opaque

* Suspended particles

* Odour: Mixture of onion and soap

3. After pouring through a strainer * Lime colour

* Translucent

* Fluidy

* Foam on the circumference

* Odour: mixture of onion and soap

4. After addition of meat tenderizer * Lime colour

* Translucent

* Fluidy

* Foam on the circumference

* Odour: mixture of onion and soap

5. After 5 minutes * Lime colour

* Translucent

* Fluidy

* Foam on the circumference

* Odour: mixture of onion and soap

6. After layering of alcohol on top * Bubbles rising to the top

* Thick layer on the top

* Thick, white stuff formed

* Three layers: 1) top layer - full of bubbles - opaque; 2) middle layer -alcohol- translucent; 3) bottom layer - original mixture - translucent

7. After 1 minute * Bubbles rising to the top

* Thick layer on the top

* Thick, white stuff formed

* Three layers: 1) top layer - full of bubbles - opaque; 2) middle layer -alcohol- translucent; 3) bottom layer - original mixture - translucent

8. Froth * Viscous, mucus-like

* White

* Soapy & full of bubbles

* Opaque

9. Onion mixture * Light yellow

* Very fluidy

* Strong onion odour

* Foam formed at the top

* Opaque

10. Dish soap * Dark green

* Very viscous

* Bubbles trapped inside it

* Foam formed at the top

* Translucent