How To Write A Lab Report In Microbiology
Essay by 24 • March 3, 2011 • 1,731 Words (7 Pages) • 3,148 Views
HOW TO WRITE AN UNKNOWN LAB REPORT IN MICROBIOLOGY
GENERAL
Unknown reports in microbiology are written in scientific format. Scientific writing is written
differently from other types of writing. The results of the exercise or experiment are what are being
showcased, not the writing. The purpose of scientific writing is not to entertain, but to inform. The
writing should be simple and easy to understand. There is a specific style that must be followed
when writing scientific reports.
Scientific writing is typically written in the passive voice. The pronouns "I", "We" and "They" are
not typically used.. For example, instead of writing "I used a TSA agar plate to isolate my
unknown," it is customary to write, "A trypticase soy agar (TSA) plate was used to isolate the
unknown."
It is also customary to write in the past tense for most of the report. This includes the introduction,
the summary, the description of the materials and methods and the results. The present tense is
reserved for the conclusions about the results. See the examples given below.
Some other general rules that should be followed are:
Microbial nomenclature: The name of the bacterium should written and spelled correctly. The name
should be italicized or underlined. Italicized is preferred. For example, Staphylococcus aureus.
The genus is capitalized but the species is not. After the full genus name is given in the paper, it can
be written as S. aureus, but still italicized. This is as long as there in no other genera in the paper
that starts with the same letter.
PARTS TO THE UNKNOWN LAB REPORT
(Note: Other than the title page, the pages of the report must be numbered)
TITLE PAGE
There should always be a title page and should include the following information:
EXAMPLE OF TITLE PAGE
Title should be centered and at the top or in the middle of the page
UNKNOWN LAB REPORT # 1
This information should be centered and at the bottom part of the title page:
UNKNOWN LETTER (OR NUMBER)
YOUR NAME
DATE (the due date)
LAB INSTRUCTOR'S NAME
COURSE NAME
SEMESTER / YEAR
SECTION NUMBER
INTRODUCTION
This section introduces the reader to the study and why the study was done. This should only be a
few sentences long.
Example: "There are many reasons for knowing the identity of microorganisms. The
reasons range from the knowing the causative agent of a disease in a patient, so as to know how it
can be treated, to knowing the correct microorganism to be used for making certain foods or
antibiotics. This study was done by applying all of the methods that have been learned so far in the
microbiology laboratory class for the identification of an unknown bacterium."
MATERIALS AND METHODS
This is where the details of the study are listed. Where did the specimen come from, and what
methods were used to identify it? Be specific, but do not re write the lab manual. One way is to
mention the names of the materials used and reference the lab manual for the procedure or method
and then continue to elaborate when necessary. See example 1.
Example 1: "An unknown labeled as letter G was given out by the lab instructor. The
methods that have been learned thus far for identifying bacteria have been applied to this unknown.
Procedures were followed as stated in the course laboratory manual by De Mers (1), unless
otherwise noted.
The first procedure that needed to be done was to streak the unknown out on a Trypticase Soy Agar
plate, using the T streak method described in the lab manual.. This needed to be done in order to test
the purity of the unknown. After the plates were incubated and grown, the morphology was
observed and recorded and a Gram stain was performed. Quality control bacteria were Gram stained
along with the unknown to make sure that the Gram stain reaction was done correctly . After
determining the Gram reaction, specific biochemical tests were performed. The biochemical tests
were chosen from the unknown identification tables that were in the lab manual. Since unknown G
was determined to be a Gram negative rod, an oxidase test was performed and the organism was
inoculated into a BCP lactose tube. Note all of these tests were performed by the methods listed in
the lab manual by De Mers (1). Table 1 lists the test, purpose, reagents and results. All of the
following tests were performed on this unknown:
1. Oxidase test
2. BCP Lactose
3. Indole
4.
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