Relationship Between Enzymes and Rate of Reaction

Relationship between Enzymes and Rate of Reaction

Enoch McAtee

Annmarie Sajeev

Ms. Reis

SBH 4U1

Date Performed: Friday, September 22, 2017

Submitted: Tuesday, September 26, 2017

Materials List

• Safety Glasses (*2)
• Ruler
• Stirring Rod
• Distilled Water
• Filter Paper Cutout  (28)
• Beef Liver Catalase (150ml for procedure 1 &80ml for procedure 2)
• Potato Catalase (80ml)
• Apple Catalase (80ml)
• H2O2 (313 ml) 3%
• Distilled Water (387 ml)
• Tweezers
• Stopwatch
• Blender
• Waste Beaker
• 2 graduated cylinder (100ml)
• 15 test tubes
• 6 250 ml beaker
• 1 300 ml beaker for Distilled Water
• Test tube rack

Procedure # 1

1. 187 ml of 3% Hydrogen peroxide was measured and poured into 100 mL graduated cylinder.  Then the hydrogen peroxide was transferred from the 100 mL graduated cylinder to 250 ml beaker
2. 261 ml of Distilled water was measured and poured into a 100 mL graduated cylinder.  Then the distilled water was poured from the graduated cylinder to a 300ml beaker.
3. 150 ml of beef liver catalase was poured into a 250 ml beaker and placed on ice.
4. 0% hydrogen peroxide was made.  112 ml of distilled water was measure using 100 ml graduated cylinder and was poured into 250 ml beaker.  
5. From the 250 ml beaker that contained 0% hydrogen peroxide, 28 ml was measured using graduated cylinder and poured into test tube.  Repeated 2 more times
6. 0.5% Hydrogen peroxide was made first.  19 ml of 3% hydrogen peroxide-from the 250 ml beaker in step # 1-was poured and measured, using one 100 ml graduated cylinder.  Simultaneously, using second 100 ml graduated cylinder, 93ml of distilled water was measured.  Both substances (hydrogen peroxide and distilled water) were poured into one 250 ml beaker.  The solution was stirred using the stirring rod, and the beaker was labelled.  Stirring rod and graduated cylinders were cleaned.  
7. 1% Hydrogen peroxide was made second.  37 ml of 3% hydrogen peroxide-from the 250 ml beaker in step # 1- was poured and measure, using one 100 ml graduated cylinder.  Simultaneously, a second 100 ml graduated cylinder measured 75 ml of distilled water from the 300 ml beaker in step # 2.  Both substances were poured from respective graduated cylinders into one 250 ml beaker.  The solution was stirred using the stirring rod, and the beaker was labelled.  Stirring rod and graduated cylinders were cleaned.  
8. 1.5 % Hydrogen peroxide was made third.  56 ml of 3 % hydrogen peroxide- from the 250 ml beaker in step # 1-was poured and measured into one 100 ml graduated cylinder.  Simultaneously, a second 100 ml graduated cylinder measured 56 ml of distilled water from the 300 ml beaker in step # 2.  Both substances were poured from respective graduated cylinders into one 250 ml beaker.  The solution was stirred using the stirring rod, and the beaker was labelled.  Stirring rod and graduated cylinders were cleaned.  
9. 2.0% of hydrogen peroxide was made fourth.  75 ml of 3 % hydrogen peroxide- from the 250 ml beaker in step # 1-was poured and measured into one 100 ml graduated cylinder.  Simultaneously, a second 100 ml graduated cylinder measured 37 ml of distilled water from the 300 ml beaker in step # 2.  Both substances were poured from respective graduated cylinders into one 250 ml beaker.  The solution was stirred using the stirring rod, and the beaker was labelled.  Stirring rod and graduated cylinders were cleaned.  
10. 28 ml of 0.5 % hydrogen peroxide was measured and poured into a 100 ml graduated cylinder, and then transferred into a test tube.  Repeated two times with new test tube each time.  Graduated cylinder was cleaned.  
11. Repeat step # 8 for 1.0 %, 1.5%, and 2.0 %.  The 15 test tubes with the respective hydrogen peroxide concentrations were labelled accordingly, and placed in test tube rack.
12. 10 ml of beef liver catalase was measured and poured into graduated cylinder and transferred into one 80 ml beaker.  
13. One filter paper cut out was selected by tweezers and dipped into the 80 ml beaker containing catalase.  The stirring rod was used to soak the filter paper in the catalase solution.  Then the stirring rod was used to stick the filter paper to its bottom.  The stirring rod was then dipped into the test tube- the side containing the filter paper pushed first into the test tube-containing 0% hydrogen peroxide.  Once it was assured that the filter paper was at the bottom of the test tube, stirring rod was taken out, and stopwatch was started.  When the filter paper reached the top, the stopwatch was stopped.  Results were documented, and products were poured into waste beaker.  Stirring rod, graduated cylinder, and the beaker used for the catalase solution was cleaned.
14. Repeat steps 10-11 for 0.5 %, 1.0%, 1.5%, and 2.0 % concentration containing test tubes.
15. The contents of the waste beaker were poured into sink.  All equipment used in the lab were cleaned, and lab station was cleared.

Procedure # 2

1. 80 ml of potato catalase was measured using 100 ml graduated cylinder and poured into 80 ml beaker.  Repeated for apple catalase and beef catalase*.
2. 1.5% Hydrogen peroxide was made.  Measured 126 ml of 3 % hydrogen peroxide using 100 ml graduated cylinder and poured into 250 ml beaker.  Measured 126 ml of distilled water using 100 ml graduated cylinder and poured into the same 250 ml beaker.  Solution was stirred using stirring rod, and the beaker was labelled by its concentration.  Stirring rod and graduated cylinders were cleaned.[pic 1]
3. From the 250 ml beaker containing 1.5 % hydrogen peroxide, 28 ml were measured and poured into 100 ml graduated cylinder, and then transferred into test tube.  Repeated 11 times.
4. Tweezers were used to select filter paper cut out.  The filter paper cut out was then placed at the bottom of the stirring rod, which was pushed into one 1.5% hydrogen peroxide containing test tube (the bottom containing the filter paper cut out was pushed first into the test tube).  Once it was assured the filter paper was at the bottom, the stirring rod was taken out and the stop watch was started.  Once the filter paper stopped rising or was at the top of the test tube, the stop watch was stopped.   The contents of the test tube was poured into waster beaker.  The stirring rod was cleaned.  Repeated 2 more times with 2 new test tubes (containing 1.5% hydrogen peroxide) and filter paper cut outs.
5. 20 ml of potato catalase- from the beaker in step #1-was measured used 10 ml graduated cylinder and poured into 80 ml beaker.  Tweezers were used to select filter paper cut out and dropped it in the 80 ml beaker (containing 20 ml of potato catalase).  Stirring rod was used to mix the filter paper in the solution.  The filter paper was placed at the bottom of the stirring rod.  Then the stirring rod was pushed into a 1.5% containing test tube (the bottom containing the filter paper cut out was pushed first into the test tube).  Once it was assured the filter paper was at the bottom, the stirring rod was taken out and the stop watch was started.  Once the filter paper stopped rising or was at the top of the test tube, the stop watch was stopped.   The contents of the test tube was poured into waster beaker.  The stirring rod was cleaned.  The contents of the 80 ml beaker containing the 20 ml of potato catalase was also poured into the waste beaker.  Repeated 2 more times.
6. Step # 5 was repeated using beef catalase and apple catalase using the remaining.
7. The contents of the waste beaker were poured into sink.  All equipment used in the lab were cleaned, and lab station was cleared.

Observations

Experiment # 1

Table # 1: Substrate Concentration 0% Effect on Rate of Reaction

Table #2:  Substrate Concentration 0.5% Effect on Rate of Reaction

Table # 3: Substrate Concentration 1.0% Effect on Rate of Reaction

Table # 3: Substrate Concentration 1.5% Effect on Rate of Reaction

Table # 3: Substrate Concentration 2.0% Effect on Rate of Reaction

Calculations

0% Concentration

CiVi=CfVf

Ci(0.03)=(0.00)(28ml)

CI=0

0 ml of H2O2; 28 ml of Water;      28 *4 =112 ml of H20