Science Lab

Problem: How are carbohydrates identified as a monosaccharide?

Hypothesis: I predict that when we add the Benedict\\\'s solution to the different types of saccharides and then boil it we will most definitally be able to determine all the information the we need to accumulate to record data resulting in the conclusion for the Benedict\\\'s solution. I always plan to see the addition of the Iodine work wonders in its own way. Both ways will help us determine factors with the unknown and known carbohydrates. I also predict that they will change colors. Especially the carbohydrates that are monosacchrides. The thicker of them will abviously be a more bland color.

Experiment:

Materials- test tubles, beakers, hot plate,++ rack, ++ holder,di. , mono. , poly. , honey, oats, apple juice, table sugar, powdered sugar.

Procedure: 1. First we got into our lab groups and gathered all the materials and set up the test tubes.

2. Then we did the first experiment by putting 30 drops of monosaccharide, polysaccharide, and disaccharide into three different test tubes. Then we added with a dropper, drops of the Benedicts solution into each test tube. Them three people in our group went over to the heater and immediately

heated our tubes in steaming water. Then brought them back so we could record the changes in color.

3. After we finished recording the data for the Benedicts solution, we washed out the test tubes. Then we went back to our station, and set the three test tubes back up. We did the same as before by adding 30 drops of each of the three sacchrides but instead of adding Benedicts solution, we added Iodine. We then took our tubes over and heated them again, and once heated and brought back, we recorded the last of our data for the test with known carbohydrates.

4. We now washed out five of the test tubes to begin our test with unknown carbohydrates. We set up our station again and aligned

each of the test tubes. We then put 20 drops of honey in test tube one, 20 drops of oats in test tube 2, 20 drops of table sugar in test tube three, 20 drops of apple in test tube four, and 20 drops of powdered sugar in the last test tube. Then we added drops of the Benedict\\\'s solution to each tube. We immediately mixed the solutions in the tubes, and recorded the data for Benedict\\\'s solution.

5. For our final experiment we once again washed all five tubes out and set up our stations. Again, we added 20 drops of each unknown carbohydrate to each of the five test tubes. Then instead of adding the Benedict\\\'s solution to the carbohydrates, we added drops of Iodine. Then we individually mixed the solutions in each test tube. After waiting for the color to develop, we recorded the data and determined what each carbohydrate was.

Conclusion:

Well, I have learned a lot

about the different types of saccharides. Not only with Mrs.Pitts wonderful help, but researching and reading out of Biology books. I have taken highly accurate notes to further

help my education about saccharides and carbohydrates. Also, by learning more and