Microbiology

Unknown Number 29

Enterobacter aerogenes

TESTS DONE

1. Gram Stain - Gram Negative Rod

2. SIM tube - motile, non-sulfur producing, indole negative

3. Simmon's Citrate tube - positive

4. Durham tubes -

a. Does ferment galactose

b. Does not ferment glucose

c. Does ferment maltose

d. Does ferment glycerol

e. Does ferment sucrose

f. Does ferment trehalose

5. Urea tube - urease negative

6. Oxidase test - does not produce cytochrome oxidase

7. Catalyse test - produced bubbles

8. Grew at 41۫C and not at 4۫C

9. Spirit Blue Agar - lipase positive

10. Lysine Decarboxylate - lysine negative

11. Nitrate Broth - nitrites produced (turned red after adding both reagents)

12. MR-VP medium -

a. Methyl red positive

b. Indole negative

c. Voges-Proskauer negative

d. Citrate utilization positive

According to Bergey's Manual, the Genus Enterobacter are straight rods, Gram negative. Most are motile and classified as facultatively anaerobic. Their